| # | Summary |
| 1. | Egg shape/size abnormal |
| Eggs small (less than 70% of wild type), large (more than 130% of wild type) or irregularly shaped | |
| 2. | Osmotic sensitivity |
| Swelling of the embryo to fill the egg shell and/or irregular granule movements | |
| 3. | P0 cortical ruffling abnormal |
| Excessive or no early cortical ruffling (wild type: wave of membrane ruffling from posterior to anterior resulting in pseudo-cleavage furrow) | |
| 4. | P0 cytoplasmic flows reduced/absent |
| Lack of granular flows towards the male PN (wild type: directional flow of yolk granules towards the male PN) | |
| 5. | P0 pseudo-cleavage furrow abnormal |
| No or excessive furrowing (wild type: covering 20-30% the width of the embryo) | |
| 6. | P0 pronuclei: size/shape abnormal |
| Size more than 30% smaller or larger than wild type (wild type: diameter approx. 25% the width of the embryo at onset of PN migration), or irregular shape of PNs | |
| 7. | P0 pronuclei: number abnormal |
| Numbers of PNs above or below 2 (wild type: a* female, one male PN) | |
| 8. | P0 pronuclear migration reduced/absent (female) |
| No or little migration of female PN towards the male PN (wild type: directional movement of the female towards the male PN) | |
| 9. | P0 pronuclear migration reduced/absent (male) |
| No migration of male PN from posterior cortex towards centre of the embryo | |
| 10. | P0 pronuclear meeting position abnormal |
| Position near the cortex or centrally (wild type: near the centre of the posterior half of the embryo) | |
| 11. | P0 pronuclear centration absent |
| Lack of centration | |
| 12. | P0 pronuclear rotation |
| Rotation takes place after PN envelope breakdown (wild type: approx. 2-3 min before Pronuclear Envelope Breakdown) | |
| 13. | P0 pronuclear envelope breakdown delayed |
| Lag time between PN meeting and PN envelope breakdown exceeds 8 min (wild type: 4-5 min) | |
| 14. | P0 spindle assembly: bipolarity abnormal |
| Lack of two visible poles, that is, two regularly shaped sites of yolk granule exclusion | |
| 15. | P0 spindle integrity abnormal |
| Irregular length (wild type: 25-30% the length of the embryo during metaphase) or thickness (wild type: 20-25% the width of the embryo at metaphase) or lack of rigidity | |
| 16. | P0 spindle elongation abnormal |
| Spindle is shorter or longer than 50-60% the length of the embryo at telophase | |
| 17. | P0 spindle rocking abnormal |
| No or excessive spindle rocking | |
| 18. | P0 spindle positioning: asymmetry abnormal |
| Aberrant spindle positioning at telophase (wild type: along the longitudinal axis with the posterior pole shifted posteriorly approx. 10-15%) | |
| 19. | P0 spindle poles abnormal |
| Irregular shape, in particular lack of flattening of posterior pole in telophase | |
| 20. | P0 cytokinesis: furrow specification abnormal |
| Fewer or more than two sites of furrowing and/or aberrant positioning (wild type: two sites, intersecting the long axis by a ratio of approx. 3:2) | |
| 21. | P0 cytokinesis: furrow ingression abnormal |
| Little or no ingression, or uneven ingression from the two sites | |
| 22. | P0 cytokinesis: completion/stability abnormal |
| Regression of the furrow | |
| 23. | P1/AB nuclear separation: cross-eyed |
| Reforming daughter nuclei stay closely attached to the central cortex | |
| 24. | AB nuclear migration abnormal |
| Time for centration of AB nucleus exceeds 7-8 min, AB nucleus migrates towards the cortex before centration (wild type: AB nucleus usually centres directly after cytokinesis) | |
| 25. | P1/AB cortical activity exaggerated |
| Excessive membrane ruffling and blebbing | |
| 26. | P1/AB nuclei: number abnormal |
| Numbers of nuclei in daughter cells below or above 1 | |
| 27. | P1/AB nuclei: size/shape abnormal |
| Size >30% smaller or larger than WT (diameter approx. 25% of AB blastomere), or irregular shape of nuclei | |
| 28. | P1 nuclear migration/rotation absent/reduced |
| Lack of migration of P1 nucleus towards posterior cortex, lack of rotation of P1 spindle before nuclear envelope breakdown | |
| 29. | P1/AB spindle assembly abnormal |
| Aberrant bipolarity or length or thickness | |
| 30. | AB spindle orientation abnormal |
| Rotation of AB spindle (wild type: AB spindle keeps orientation, whereas P1 spindle rotates) | |
| 31. | P1/AB asynchrony of divisions abnormal |
| Delay between AB and P1 cleavage furrow initiation is either shorter than 2 min or exceeds 5 min (wild type: approx. 2-3 min) | |
| 32. | P1/AB cytokinesis abnormal |
| Aberrant furrow initiation or ingression or completion | |
| 33. | Four-cell stage: cross-eyed |
| Reforming daughter nuclei stay closely attached to the central cortex | |
| 34. | Four-cell stage nuclei: size/shape abnormal |
| Irregular size and/or shape of nuclei in daughter cells | |
| 35. | Four-cell stage configuration abnormal |
| PAR-like configurations of blastomeres | |
| 36. | Tetrapolar spindle |
| Four poles, visible by exclusion of yolk granules | |
| 37. | Yolk granules: density reduced |
| Reduction more than 30% | |
| 38. | Yolk granules: size abnormal |
| Aberrant size of individual or all granules | |
| 39. | Areas devoid of yolk granules |
| Aberrant cytoplasmic structures excluding yolk granules | |
| 40. | Polar bodies abnormal |
| Aberrant number (fewer or more than two) or size (matches or exceeds size of early PNs) or internalization of polar bodes | |
| 41. | Unclear: multinucleate |
| Aberrant numbers of nuclei | |
| 42. | Overall pace of events slow |
| Time span between pronuclear meeting and initiation of AB cleavage furrow exceeded 30 min (wild type: 18-22 min) | |
| 43. | Number/age of embryos atypical |
| Absence or reduction of one-cell and two-cell stage embryos, suggesting reduced fertility of the parent worm | |
| 44. | Meiotic arrest |
| No visible PNs, few or no cytoplasmic events, embryo often fills egg shell | |
| 45. | Other |
| Any other observation differing from wild-type events | |
| 46. | Inadequate test |
| Technical inadequacy (focusing, coverage of recordings, etc.) |